Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells

Figure 3

Generation and characterization of 3D retinal organoids from U-hiPSCs. (a) Example image of retinal organoids with neural retina (NR) and retinal pigment epithelium (RPE) from UE017-hiPSCs on W6 after differentiation. Scale bar = 100 μm. (b) Neural retina comprised mostly retinal progenitor cells expressing CHX10 and cell proliferative marker MCM2 on W6 after differentiation. Scale bars = 50 μm. (c, d) The polarity of NRs with FIBRONECTIN⁺ cells (c) located at the basal side while a tight junction marker ZO-1⁺ cells (d) at the apical side. Scale bars = 50 μm.

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