Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells
Gene expression profiles of O-iPS cells. (a) qPCR analysis for the indicted transcript levels in 3 F-iPS clones, 3 O-iPS clones, sorted GFP+ osteoblasts, ESCs, and MSCs. Individual mRNA expression levels were normalized to Gapdh with fold change relative to dermal fibroblasts which is arbitrary set to 1. (b) Heatmap of log fold change (Log2 FC) values for expression levels of the indicated transcripts in fibroblasts, MSCs, ESCs, GFP+ osteoblasts, F-iPS, and O-iPS clones. (c) Immunofluorescence of ESCs, O-iPS clone 6, and F-iPS clone 4 for antibodies against SSEA1 and OCT4. Scale bar: 100 μm. Three independent experiments are represented in (a). Data are expressed as means ± SEM. ;.