Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses

<div>Epigenetic state of ASC<sub>CTRL</sub> and ASC<sub>EMS</sub> cultured under adipogenic conditions. TEM images of nuclei (a) and their higher magnification showing heterochromatin underneath the nuclear envelope. ASC<sub>EMS</sub> was characterized by a breakdown of heterochromatin associated with the inner nuclear membrane. Immunofluorescence staining for genomic distribution of 5-mC and 5-hmC (b) was performed in order to evaluate DNA methylation status. Using RT-PCR, expression of TET2 (c), TET3 (d), and DNMT1 (e) was evaluated. Obtained results indicate increased DNA methylation in ASC<sub>EMS</sub>. Results are expressed as mean ± SD. 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Stem Cells International

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Figure 5

Figure 5: Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses 

Figure 5 | Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses 