The Potential of Menstrual Blood-Derived Mesenchymal Stem Cells for Cartilage Repair and Regeneration: Novel Aspects

Table 2

Evidence of MenSC chondrogenic differentiation.

Method	Visualization with	Growth factors/other components used	Duration	Results	Reference
2D	Alcian blue	TGF-β3	14–20 days	Alcian blue positive	[60]
	Alcian blue, RT-PCR for collagen type II and Sox9	TGF-β3, BMP-6, fibronectin-coated	21 days	Alcian blue positive, collagen type 9 and Sox9 positive, collagen type II negative	[24]
	IHC collagen type II antibody	TGF-β3, BMP-6	21 days	Collagen type II positive	[52]
	Alcian blue	TGF-β3	14 days	Alcian blue positive	[20]
	Alcian blue	TGF-β3	21 days	Alcian blue positive	[53]
3D	Alcian blue, IHC with collagen type II antibody	TGF-β3, IGF-1, nanofibrous scaffolds	4 weeks	Alcian blue and collagen type II positive	[11]
	Alcian blue, IHC with collagen type II and type I antibodies	TGF-β3, nanofibrous scaffolds	3 weeks	Alcian blue and collagen type II positive, collagen type I negative	[23]
	Alcian blue	TGF-β, BMP-6	21 days	Alcian blue positive	[61]
	IHC with collagen type II antibodies	TGF-β3, IGF-1	4 weeks	Collagen type II positive	[62]
	Safranin O, collagen type II RNA gene analysis	TGF-β3	21 days	Safranin O and collagen type II gene positive	[22]