Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress

Figure 6

Preliminary experiments to establish the appropriate IL-6 concentration for in vitro testing. Cells were seeded at a density of 3 × 10³/cm². After 1 day in cell culture, cells were subjected to 4-hour simulated acute “ischemia” and 4-hour reoxygenation (AcS). Cell numbers were quantified by Hoechst-based nucleated cell counts. Cell survival was calculated by dividing cell numbers from experimental groups by control group. and .