In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium

Figure 3

Histograms of nonclassical MSC marker expression in pMSCs from selected donors. The expression of a panel of nonclassical MSC markers in unsorted pMSCs from different donors was investigated after P4 in a human protein-based xeno-free medium. Histograms of FACS analysis showed the positive expression of CD200, CD274, and CD140b in pMSCs from donor #6 (a) and the positive expression of CD200, CD274, and CD146 in those from donor #10 (b). The rest of nonclassical markers were negative. Dotted line: background fluorescence intensity of a control antibody, solid line: fluorescence intensity of an anti-non-classical MSC marker antibody staining.