In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium

Table 2

Comparison of classical MSC marker expression of pMSCs after P4 in FBS standard medium with that after P4 in human protein (xeno-free) medium.

Donor no.	1		2		3		4		5
Medium	Standard	Xeno-free	Standard	Xeno-free	Standard	Xeno-free	Standard	Xeno-free	Standard	Xeno-free
CD14	214	117	155	110	207	140	189	156	135	101
CD29	16,435	27,096	27,172	49,100	48,154	70,848	50,064	38,239	45,174	40,875
CD34	143	135	153	162	230	190	106	163	192	104
CD44	34,600	16,572	20,185	39,866	63,599	53,580	21,398	43,814	50,905	50,764
CD45	147	151	132	117	1184	548	135	158	175	178
CD73	1126	1265	1563	5656	1500	5382	519	1941	2444	2424
CD79a	145	164	171	136	535	246	248	128	123	189
CD90	17,962	15,247	8252	10,723	14,105	14,383	8391	19,469	23,128	36,576
CD105	189	203	198	142	443	228	532	238	253	244
CD146	377	196	243	156	394	246	148	144	199	169
CD166	704	495	530	409	1462	2944	567	1611	5019	4895
CD271	144	161	164	133	282	215	92.4	158	126	186
HLA-DR	138	143	176	141	329	127	1077	150	182	189
SSEA	163	24.1	224	117	334	137	301	119	210	195
Stro-1	158	17.6	194	126	338	135	279	134	203	222

Data were presented by median of fluorescence intensity (MFI). Background MFI: 224 ± 50.7.