Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs

Figure 2

Cell viability and cell cycle analyses of MCF7 cells exposed to hUCMSC-CM (a) Effect of hUCMSC-CM on cell viability of MCF7 cells. x-axis represents days MCF7 cells exposed to hUCMSC-CM. y-axis represents cell viability rate. Cell viability rate = (the average OD of treatment group/the average OD of control group)100%. Results were measured using MTS proliferation method. Experiments were carried out in triplicate and repeated 3 times. . (b) Flow cytometric analysis for cell cycle of MCF7 cells exposed to hUCMSC-CM. Treatment cells were collected after 48 h, cultured in hUCMSC-CM, and stained with PI. (c) Quantitative analysis for cell cycle. Data were presented as mean ± SD of three independent experiments. hUCMSCs: human umbilical cord mesenchymal stem cells.

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