Research Article
Establishment and Identification of a CiPSC Lineage Reprogrammed from FSP-tdTomato Mouse Embryonic Fibroblasts (MEFs)
Table 1
Details of primers used for qRT-PCR analysis of selected genes used in the PCR experiments.
| | Gene name | Primer sequences | Annealing T (°C) | GenBank accession number |
| | Oct4 | F: 5-CATTGAGAACCGTGTGAG-3 R: 5-TGAGTGATCTGCTGTAGG-3 | 60°C | NM_013633.3 | | Sox2 | F: 5-AGGGCTGGGAGAAAGAAGAG-3 R: 5-CCGCGATTGTTGTGATTAGT-3 | 60°C | NM_011443.4 | | Sall4 | F: 5-CTAAGGAGGAAGAGGAGAG-3 R: 5′-CAAGGCTATGGTCACAAG-3 | 60°C | NM_175303.4 | | Nanog | F: 5-CTCAAGTCCTGAGGCTGACA-3 R: 5-TGAAACCTGTCCTTGAGTGC-3 | 60°C | NM_028016.3 | | Rex1 | F: 5-CAGCCAGACCACCATCTGTC-3 R: 5-GTCTCCGATTTGCATATCTCCTG-3 | 60°C | NM_009556.3 | | Dppa5a | F: 5-CCGTGCGTGGTGGATAAG-3 R: 5-GCGACTGGACCTGGAATAC-3 | 60°C | NM_025274.3 |
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