Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl₄-Induced Liver Injury through Antioxidant Effect

Figure 6

hucMSC-Ex inhibits oxidative stress in CCl₄-injured L02 cells. (a) ROS production in CCl₄-injured L02 cells treated with different doses of hucMSC-Ex (0 particles/ml, 4 × 10⁸ particles/ml, and 16 × 10⁸ particles/ml). Original magnification 200x. (b) Percentage of DCF-positive hepatocytes in hucMSC-Ex treated L02 cells using imaging flow cytometer. Data showed that hucMSC-Ex significantly decreased percentage of DCF-positive hepatocytes. (c) Fluorescence intensity of DCF-positive L02 cells was significantly decreased by hucMSC-Ex treatment. DCF fluorescent values are means ± SD. (; ). (d) Immunohistochemistry staining of PCNA in hucMSC-Ex-treated L02 cells. Percentage of PCNA-positive L02 cells and PCNA expression was enhanced in the hucMSC-Ex group compared with that in the PBS group. Original magnification 200x. (e) Western blot quantification of activated caspase 3 and Bcl2. hucMSC-Ex induced Bcl2 expression and inhibited activated caspase 3 expression in CCl₄-injured L02 cells. (f) Experimental model design of hucMSC-Ex in CCl₄-induced liver tumor development.

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