Guanylate-Binding Protein 1 Promotes Migration and Invasion of Human Periodontal Ligament Stem Cells

<div>Depletion of GBP1 suppressed IFN-<i>γ</i>-induced migration and invasion of PDLSCs. The knockdown efficiency was confirmed by RT-qPCR and Western blot (a and b). To examine the effect of GBP1 knockdown on migration of PDLSCs, wound healing assay was performed using GBP1-depleted PDLSCs (siGBP1) and scrambled siRNA-transfected cells (siSCR). Representative images were acquired at the initial time point (0 h) and after 24-hour migration (24 h), respectively. The upregulation of migration induced by IFN-<i>γ</i> treatment was inhibited by GBP1 knockdown (c and d). Bar indicates 500 <i>μ</i>m in (c). The upregulation of invasion induced by IFN-<i>γ</i> treatment was also inhibited by GBP1 knockdown as revealed by transwell invasion assay (e and f). 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Stem Cells International

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Figure 2

Figure 2: Guanylate-Binding Protein 1 Promotes Migration and Invasion of Human Periodontal Ligament Stem Cells 