Stem Cells International / 2018 / Article / Fig 2

Research Article

Guanylate-Binding Protein 1 Promotes Migration and Invasion of Human Periodontal Ligament Stem Cells

Figure 2

Depletion of GBP1 suppressed IFN-γ-induced migration and invasion of PDLSCs. The knockdown efficiency was confirmed by RT-qPCR and Western blot (a and b). To examine the effect of GBP1 knockdown on migration of PDLSCs, wound healing assay was performed using GBP1-depleted PDLSCs (siGBP1) and scrambled siRNA-transfected cells (siSCR). Representative images were acquired at the initial time point (0 h) and after 24-hour migration (24 h), respectively. The upregulation of migration induced by IFN-γ treatment was inhibited by GBP1 knockdown (c and d). Bar indicates 500 μm in (c). The upregulation of invasion induced by IFN-γ treatment was also inhibited by GBP1 knockdown as revealed by transwell invasion assay (e and f). Bar indicates 100 μm in (e). ; .

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