Research Article

Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System

Figure 2

Increase in migration of rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) cocultured with LPS-stimulated microglia. (a) Gene network related to cell migration was constructed, and cellular function was predicted algorithmically using Ingenuity Pathway Analysis. Red and green areas indicate up- and downregulated genes, respectively. Differentially expressed genes were obtained from microarray data (>1.2 fold change). (b) quantitative RT-PCR analysis of gene expression related to cell migration in rBM-MSCs cocultured with LPS-stimulated microglia compared to rBM-MSCs cocultured with microglia. Data represent the mean of three independent experiments. () versus rBM-MSCs cocultured with microglia.
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