Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System

Figure 4

Inflammation-related gene expression variation in LPS-stimulated microglia cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs). (a) In vitro reverse coculture experimental design. Three different conditions were used (group 1–3): group 1, control (microglia only); group 2, LPS-stimulated microglia; and group 3, LPS-stimulation with rBM-MSC coculture. (b) Canonical pathway analysis was constructed algorithmically using Ingenuity Pathway Analysis based on microarray data. Bars indicate canonical pathways containing genes with significantly altered expression. Bar graph colours from blue (inhibition) to orange (activation) represent gene activity of the corresponding pathway according to -score. (c) Heat map of genes related to inflammation with altered expression levels. Gene expression values are coloured from green (downregulated) to red (upregulated).

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