EZH1 Is Associated with TCP-Induced Bone Regeneration through Macrophage Polarization

Figure 1

Osteogenic effect and gathering of macrophages at the healing defects filled with TCP. (a) (A–D) Representative sections of hematoxylin-eosin staining. Macrophages are indicated by red arrows. (E) Representative sections of immunohistochemistry staining with F4/80 antibody. Macrophages are indicated by red arrows. (F) Representative sections of immunohistochemistry staining with CD206 antibody. M2 macrophages are indicated by red arrows. (A,C) Scale bar = 50 μm; (B, D, E, and F) scale bar = 20 μm. (b) Relative mRNA expression of ALP, OCN, OSX, and Runx2 of HBMSC stimulated by control medium, supernatant of control Thp1, LPS, IL-4, and TCP extract and supernatant of TCP-induced Thp1. (c) Alizarin red staining, ALP staining, and quantification of HBMSC stimulated by control medium, supernatant of control Thp1, LPS, IL-4, and TCP extract and supernatant of TCP-induced Thp1. (d) Quantification of the areas of alizarin red staining and ALP staining was measured by ImageJ software. , , , and .

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