Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5a/β-Catenin Signaling Pathway

Figure 3

Validation of Wnt/β-catenin expression in MSCs-H or MSCs-L. (a) Cells cultured in adipogenic medium for 14 days. Expression of Wnt family members (Wnt1, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt10b) in MSCs-H or MSCs-L was determined with qPCR (mean ± SD, , ). Wnt5a expression showed a significant decline during the adipogenic induction of M-H (black box). The expression levels of these genes were normalized to those of β-actin in control cells, which was defined as 1. (b) Intracellular distribution and expression of β-catenin (green) assessed by quantifying the percentage of positively stained cells. Nuclei were stained with DAPI (blue). The merged image is an overlay of the DAPI and catenin images (scale bar = 50 μm, mean ± SD, , ). C: control; I: induction.

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