High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets

Table 7

Reduced quadratic mixture model for the number of live cells.

Additives	F value	p value prob > F	Effect
Glycerol (10%)	14.41	0.0004	+
Lactic acid	2.87	0.0964	−
Glycerol + aspirin (400 μg/ml)	5.89	0.0187	−
Glycerol + ATP (200 μM)	12.34	0.0009	−
Glycerol + lactic acid (40 mM)	15.78	0.0002	−
Glycerol + melatonin (2 nM)	8.72	0.0047	−

F value: test for comparing model variance with residual variance; p value prob > F: probability of observed F value if the null hypothesis is true (small value call for rejection of the null hypothesis) Parentheses indicate maximal tested concentrations of the relevant additives and not necessarily the optimal levels.