TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton
Molecular phenotype of TGFβ1-treated hMSCs. (a) Hierarchical clustering of genes that were differentially expressed in TGFβl-treated and untreated control (CNT) hMSCs. Rows represent individual gene expression for duplicate treated and untreated samples, as indicated. Columns represent individual transcripts. Relative expression levels are presented colorimetrically, according to the scale shown in the color bar. (b) Pie chart showing the pathways with the highest enrichment of genes significantly upregulated in TGFβ1-treated cells. (c) qRT-PCR validation of selected genes that were upregulated in the microarray data (,; ). Cells treated with vehicle (DMSO) were used as controls.
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