Research Article

Low Magnitude of Compression Enhances Biosynthesis of Mesenchymal Stem Cells towards Nucleus Pulposus Cells via the TRPV4-Dependent Pathway

Figure 2

Cell viability of encapsulated MSCs in the IPN hydrogel. (a–d) A fluorescent Live/Dead staining for the MSC-encapsulated hybrids treated with free swelling (FS), compressive loading at magnitude of 5% (5%), compressive loading at magnitude of 10% (10%), and compressive loading at magnitude of 20% (20%) (magnification: 40x). (e) Quantification of NPC percent (Live/Dead) for the fluorescent Live/Dead staining. (f) Measurement of the DNA content of MSC-seeded hybrids treated with free swelling and compressive loading at magnitudes of 5%, 10%, and 20%. Data were expressed as means ± SD (). and .
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