Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells

Figure 2

hES cells derived on hFFs and cultured to p9 on LN521. All five cell lines transferred from hFFs onto LN521 exhibited normal hES cell morphology (a). Immunofluorescence analysis of NANOG (b), POU5F1 (c), SOX2 (d), SSEA4 (e), and TRA-1-60 (f) expression, shown for HS380 as a representative cell line, revealed the presence of pluripotency markers after p9 on LN521 (red staining: NANOG, POU5F1, and SOX2; green staining: SSEA4 and TRA-1-60; and blue staining: DAPI). Scale bars: 50 μm. Immunofluorescence analysis of the expression of differentiation markers for endoderm (AFP) (g), ectoderm (TUJ1) (h), and mesoderm (alpha SMA) (i), shown in HS380 as a representative cell line, demonstrates the potential of hES cells cultured on LN521 to differentiate spontaneously into the three germ layers (green staining: AFP, alpha SMA and TUJ1; blue staining: DAPI). Scale bars: 50 μm.