Treatment of Full-Thickness Rotator Cuff Tendon Tear Using Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Polydeoxyribonucleotides in a Rabbit Model

Table 1

Semiquantitative score of histological findings, immunoreactivity of stain, and motion analysis according to treatment groups at 4 weeks after injection.

	Groups (injection regimens)
	0.2 mL NS (G1-SAL)	0.2 mL PDRN (G2-PDRN)	0.2 mL MSC (G3-MSC)	0.2 mL MSC + 0.2 mL PDRN (G4-MSC + PDRN)
Histological score
MTS	0.0 ± 0.0	0.9 ± 0.56	2.2 ± 0.42	3.0 ± 0.0
Anti-type collagen 1	0.2 ± 0.42	1.1 ± 0.31	2.1 ± 0.31	3.0 ± 0.0
BrdU	0.88 ± 0.84	1.54 ± 1.13	2.05 ± 1.16	2.54 ± 1.11
PCNA	1.27 ± 0.91	2.10 ± 1.07	2.70 ± 1.08	3.11 ± 1.00
VEGF	1.27 ± 0.94	2.00 ± 1.07	2.64 ± 0.92	3.18 ± 0.79
PECAM-1	1.68 ± 0.93	2.22 ± 0.92	2.86 ± 0.98	3.36 ± 0.75
Motion analysis
Walking distance (cm)	4728.37 ± 137.27	5416.62 ± 323.27	6343.62 ± 213.57	6932.37 ± 107.74
Fast walking time (%)	5.62 ± 1.42	8.32 ± 0.34	10.33 ± 2.48	12.07 ± 1.77
Mean walking speed (cm/sec)	6.3 ± 0.57	8.36 ± 0.39	9.62 ± 1.78	12.3 ± 1.13

Values are the mean ± SD. The immunoreactivity of MTS and anti-type 1 collagen antibody stain and proportion of BrdU-, PCNA-, VEGF-, and PECAM-1-positive cells were scored as detailed in Materials and Methods. NS: normal saline; MSC: human umbilical cord blood-derived mesenchymal stem cell; PDRN: polydeoxyribonucleotide; G1-SAL: 0.2 mL normal saline group; G2-PDRN: 0.2 mL PDRN; G3-MSC: 0.2 mL UCB-MSCs; G4-MSC + PDRN: 0.2 mL UCB-MSCs with 0.2 ml PDRN; MTS: Masson’s trichrome stain; BrdU: bromodeoxyuridine; PCNA: proliferating cell nuclear antigen; VEGF: vascular endothelial growth factor; PECAM-1: platelet endothelial cell adhesion molecule. one-way ANOVA, Tukey’s post hoc test among groups.