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Stem Cells International
Volume 2018 (2018), Article ID 7231739, 12 pages
https://doi.org/10.1155/2018/7231739
Research Article

Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response

1Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China
2Department of Orthopaedics, Tongren Hospital of Wuhan University, Wuhan 430060, China
3Department of Surgery, Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China
4Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China

Correspondence should be addressed to Zhanghua Li; moc.nuyila@999999hzl and Hao Lin; moc.361@rekrowgninup

Received 25 December 2017; Accepted 22 February 2018; Published 16 April 2018

Academic Editor: Yong-Ping Bai

Copyright © 2018 Mingyan Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The extracellular microvesicles (MVs) are attracting much attention because they are found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator in tailoring the differentiation potential of mesenchymal stem cells (MSCs). However, the effect of DXM on the paracrine signaling of MSCs remains unknown. To this point, we aimed to explore the role of DXM in regulating the paracrine activity of MSCs through evaluating the release and function of MSC-MVs, based on their physicochemical characteristics and support on osteogenic response. Results showed that DXM had no evident impact on the release of MSC-MVs but played a pivotal role in regulating the function of MSC-MVs. MVs obtained from the DXM-stimulated MSCs (DXM-MVs) increased MC3T3 cell proliferation and migration and upregulated Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), and osteopontin (OPN) expression. The repair efficiency of DXM-MVs for femur defects was further investigated in an established rat model. It was found that DXM-MVs accelerated the healing process of bone formation in the defect area. Thus, we conclude that using DXM as stimuli to obtain functional MSCs-MVs could become a valuable tool for promoting bone regeneration.