Research Article

Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

Figure 1

Characterization of endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood. (a) Representative image displayed the cobblestone morphology of ECFCs colony (×40). Monocytes were isolated from umbilical cord blood and cultured in dishes. The cobblestone-like colony was shown after 7 days. (b) Flow cytometry analysis for CD14, CD31, CD34, CD45, CD144, and KDR in ECFCs. Black-lined histograms represent isotype-matched controls. Cells stained with fluorescent antibodies are overlaid in solid grey histograms. (c) Representative image of capillary-like structures formed by ECFCs on matrigel (×100). ECFCs were seeded on matrigel-coated culture plate, and the self-assembled capillary-like network was shown on matrigel after 2 hours.
(a)
(b)
(c)