Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System

Figure 3

Schematic of temporal and spatial control of epigenome editing. The semicircles labeled with (A) and (B) represent a protein pair. The magenta comb-like lines represent guide RNA sequences. (A) is bound to dCas9, which would be directed by the guide RNA to the DNA target. (B) is bound to the epieffector. (a) Upon stimulation of light or chemicals, (A and B) would pair with each other thus bringing the dCas9 and epieffector together to achieve site-specific epigenome editing at a given time point. (b) Another strategy is splitting the dCas9 into two parts, each of which is bound by protein (A) or (B). Upon light or chemical stimulation, (A) and (B) would gather together to reconstruct an intact dCas9-epieffector complex to achieve site-specific epigenome editing at a given time point.

(a)

(b)