Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α

Figure 3

RT-qPCR analysis showed that AZM promotes the osteogenic differentiation of human PDLSCs and impacts the mRNA levels of epigenetic-related genes. Quantitative real-time PCR analysis of RUNX2, BSP, OCN, KDM2A, KDM2B, and EZH2. PDLSCs were treated with TNF-α and AZM as indicated. The top three images show the mRNA levels of (a) RUNX2, (b) BSP, and (c) OCN in cells treated with 1 μg/ml, 10 μg/ml, and 20 μg/ml AZM, respectively. The middle three images show the mRNA levels of (d) RUNX2, (e) BSP, and (f) OCN treated with 20 ng/ml TNF-α and 1 μg/ml, 10 μg/ml, or 20 μg/ml AZM. The mRNA levels of (g) EZH2, (h) KDM2A, and (i) KDM2B are shown in the bottom three images. PDLSCs were treated with 20 ng/ml TNF-α and 10 μg/ml AZM or with 20 ng/ml TNF-α and 20 μg/ml AZM. indicates significant differences. The data are presented as means ± SD.

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