Research Article

Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation

Figure 1

Effect of the conditioned medium from MSCs on PSL-induced neuropathic pain-like behaviors. The nociceptive thresholds were assessed in the ipsilateral paw of each mouse before (b) and after the PSL surgery, performed at time zero. (a) Thermal nociceptive threshold: the axis of ordinates represents the time (seconds) the animal takes to withdraw its paw. (b) Mechanical nociceptive thresholds: ordinates represent the filament weight (g) in which the animal responds in 50% of presentations. Sham group represents mice without neuropathy, in which the sciatic nerve was exposed but left intact. Seven days after PSL mice were treated (arrow) by endovenous route with bone marrow-derived mesenchymal cells (MSCs; 1 × 106/100 μl), conditioned medium from MSCs (CM; 100 μl) or vehicle (control medium; 100 μl). Gabapentin (70 mg/kg), the reference drug, was administered twice daily by oral route for six consecutive days (7 to 12 day). For gabapentin group, nociceptive threshold evaluations were made one hour before and one hour after the first treatment day. Data are expressed as means ± SEM; mice per group. Significantly different from the vehicle-treated group (); significantly different from the MSC and CM groups (). Two-way ANOVA followed by the Bonferroni’s test.
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