Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage Subsets

Figure 1

Identification of M1 and M2 macrophages. (a) (A) Morphology of macrophages as assessed by light microscopy on day 7 of cell culture (×100); (B) flow cytometry showing that the proportion of CD11b-positive cells was greater than 95% on day 7 of cell culture; (C) macrophages after phagocytic neutral red staining (×400). (b) Expression of CD80 and CD163 on macrophages after treatment with LPS + IFN-γ and IL-4 for 24 hours as assessed by flow cytometry. (c) Expression of iNOS and Arg-1 in macrophages after treatment with LPS + IFN-γ and IL-4 for 24 hours as assessed by Western blot.

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