Comparison of Immunological Characteristics of Mesenchymal Stem Cells from the Periodontal Ligament, Umbilical Cord, and Adipose Tissue

Figure 6

The effects of IFN-γ on the expression of HLA-ABC and HLA-DR. PDLSCs, UC-MSCs, and AD-MSCs at passage five were cultured without IFN-γ or with 1 or 10 ng/mL IFN-γ for 48 hours. Subsequently, cells were stained for (a) HLA-ABC and (d) HLA-DR, and the MFI values (b and e) and positive cell rates (c and f) were analyzed. Black histograms indicate negative controls, and red histograms represent MSCs stained with the indicated antibodies. All experiments were performed independently at least three times. The graph shows the means and SEMs. values were obtained by ANOVA followed by Tukey’s post hoc test. and ns: not significant.

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