Production of Adult Human Synovial Fluid-Derived Mesenchymal Stem Cells in Stirred-Suspension Culture

Figure 4

(a) Effect of agitation rate on the growth of SF-MSCs on Cytodex 3. Six spinner flasks were stirred continuously at 40 rpm for the first 24 hours. After 24 hours, the agitation rate in two spinner flasks was increased to 60 rpm and in another two was increased to 80 rpm. Data were collected in duplicate; error bars represent the range of data collected. (b) Effect of feeding on cell growth. In the cyclic fed batch condition, a 50% medium change was performed on days 3, 6, and 9, indicated by the arrows. No medium replenishments were made in the batch condition. Data were collected in duplicate; error bars represent the range of data collected.

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(b)