Production of Adult Human Synovial Fluid-Derived Mesenchymal Stem Cells in Stirred-Suspension Culture

Figure 5

Serial passaging of SF-MSCs on Cytodex 3 microcarriers using the improved protocol. Cells were serially passaged 3 times. Each passage lasted 6 days. (a–c) Photomicrographs showing microcarriers from the last day of culture for three consecutive passages. There are fewer cells and a larger proportion of unoccupied beads with each subsequent passage. Photomicrographs were taken at 10x magnification. Scale bars represent 200 μm. (d) Serial passaging of SF-MSCs on Cytodex 3. Lower maximum cell densities were achieved with each subsequent passage. A 50% medium replenishment was performed on days 3, 9, and 15, as indicated by the arrows. Data were collected in duplicate; error bars represent the range of data collected.

(a)

(b)

(c)

(d)