Research Article
Expansion of Gammadelta T Cells from Cord Blood: A Therapeutical Possibility
| | Target antigen | Fluorophore | Clone | Company, address |
| | CD28 | FITC | CD28.2 | BD Bioscience
Franklin Lakes, NJ, USA | | CD56 | FITC | NCAM16.2 | | CD69 | FITC | FN50 | | CD94 | FITC | HP-3D9 | | CD95 | FITC | DX2 | | PD-1 | FITC | MIH4 | | PD-1 | BV421 | MIH4 | | TCRαβ | FITC | T10B9.1A-31 | | CD8 | FITC | SK1 | | CD8 | APC Cy7 | SK1 | | CD8 | V500 | RPA-T8 | | CD45RO | APC | UCHL1 | | CD4 | Alexa Fluor 700 | RPA-T4 | | CD3 | PE | UCHT1 | | CD3 | V450 | UCHT1 | | CD3 | BV510 | UCHT1 | | CD19 | PE | HIB19 | | CD27 | PE | M-T271 | | CD27 | BV421 | M-T271 | | CCR7 | PE-Cy7 | 3D12 | | TCRγδ | FITC | IMMU510 | Beckman Coulter Inc.
Indianapolis, IN, USA | | Cd152/CTLA-4 | FITC | A3.4H2.H12 | LifeSpan Biosciences
Seattle, WA, USA | | CD223/LAG-3 | FITC | 17B4 | | CD366/TIM-3 | APC | F38-2E2 | | TCR Vδ2 | FITC | B6 | BioLegend
San Diego, CA, USA | | TCR Vγ9 | FITC | B3 | | TCR Vδ1 | FITC | TS8.2 | Thermo Fisher Scientific
Waltham, MA, USA | | TCRγδ | PE | REA591 | Miltenyi
Bergisch Gladbach, Germany | | Apoptosis and viability | | 7AAD | | | BD Biosciences | | Annexin V | FITC | | | Annexin V | APC | | Immunotools GmbH
Friesoythe, Germany |
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7-AAD: 7-aminoactinomycin; APC: allophycocyanin; CD: cluster of differentiation; FITC: fluorescein isothiocyanate; PE: phycoerythrin.
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