Research Article
STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation
Figure 2
Generation of inducible STAT3 KO and STAT3 CA ESCs. (a) Schematic chart for selection of inducible STAT3 KO and STAT3 CA ESCs. (b) Ablation of STAT3 genomic DNA in inducible STAT3 KO ESCs was detected as a 480 base pair fragment. The hearts from the cardiac-specific STAT3 KO mice (C/F/D) (lane 1, both STAT3 alleles were deleted), heterozygous (C/F/+) (lane 2, one wild-type STAT3 allele and one deleted STAT3 allele), and STAT3flox/flox mice (F/F) (lane 3, both STAT3 alleles with two loxP sites inserted) were used. Inducible Cre (iCre) (lane 4, STAT3 was deleted after Dox induction) and STAT3flox/flox (F/F) ESCs (lane 5, both STAT3 alleles with two loxP sites inserted) were used.
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