STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

Figure 4

pSTAT3 and STAT3 protein expressions can effectively respond to Dox induction. (a) In pTRE-CreCh/pEF1aTetR ESCs, after the Dox induction, pSTAT3 was reduced and undetected after 48 hours induction, while STAT3 was decreased but still detectable after 48 hours. (b) In pTRE-STAT3 CA/pEF1aTetR ESCs, pSTAT3 and STAT3 were rapidly upregulated after 24 hours Dox induction. (c) In pTRE-STAT3 CA/pEF1aTetR ESCs, after the Dox induction, flagged-STAT3 CA expression was detected, which indicated that CA-STAT3 was expressed in these cells.

(a)

(b)

(c)