The Effects of Conditioned Medium Derived from Mesenchymal Stem Cells Cocultured with Hepatocytes on Damaged Hepatocytes and Acute Liver Failure in Rats

<div>Therapeutic potential of MSC-H-CM for D-galactosamine-induced ALF in rats. (a) and (b) AST and ALT levels (a) release levels and total bilirubin (b) in peripheral blood samples collected 72 hours after the first administration of each type of CM (<span class="nowrap"><svg height="8.55521pt" id="M29" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 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(c–g) Histopathological recovery from D-galactosamine-induced ALF after treatment with each CM. Hematoxylin and eosin staining revealed massive necrosis and hepatic lobule damage in the rat liver after 24 hours of D-galactosamine administration (c). Cell necrosis was completely suppressed in the livers of rat treated with H-CM (d), MSC-CM (e), MSC-H-CM (f), and MSC-CM + H-CM (g) at 7 days after CM injection, compared with those treated with NCM (h). Bar = 50 <i>μ</i>m.</div>

Stem Cells International

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Figure 4

Figure 4: The Effects of Conditioned Medium Derived from Mesenchymal Stem Cells Cocultured with Hepatocytes on Damaged Hepatocytes and Acute Liver Failure in Rats 

Figure 4 | The Effects of Conditioned Medium Derived from Mesenchymal Stem Cells Cocultured with Hepatocytes on Damaged Hepatocytes and Acute Liver Failure in Rats 