Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation

Figure 2

Induction treatment of human adipose-derived stem cells into the retinal lineage. (a) A schematic diagram of the induction treatment of human ASCs into the retinal lineage. Human ASCs were first aggregated in IM1 for 3 days and cultured on Matrigel-coated plates for 7 days in IM2 and 14 days in IM3. Notch signaling activation and inhibition were mediated by JAG1 and DAPT, respectively. The control cells were cultured with DMEM/F12 + 10% FBS for 24 days. (b) The morphology of human ASCs after retinal induction treatment at different time points. Upper: noninduction group (control). Lower: regular induction group. The cells in the regular induction group at Day 24 exhibited a neural-network-like morphology with condensed cell bodies, while the cells in the control group remained in a fibroblast-like shape. Scale bar: 100 μm.

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