Notch Signaling Activation Enhances Human Adipose-Derived Stem Cell Retinal Differentiation

Figure 5

Retinal lineage marker expression in retinal-induced human adipose-derived stem cells upon Notch signaling modulation. (a) Gene expression analysis for markers upon Notch signaling modulation: Notch signaling effector HES1 and Notch receptor NOTCH1; retinal progenitor cell markers PAX6 and NES; photoreceptor cell markers CRX, NRL, and RHO; retinal ganglion cell markers ATOH7 and TUBB3. Relative fold change was compared to the regular induction group at Days 17 and 24 ( and ). (b) Immunoblotting analysis for the retinal progenitor marker (PAX6) and photoreceptor marker (RHO). GAPDH was used as the housekeeping gene for normalization ( and ). (c) Immunofluorescence analysis of PAX6, CRX, POU4F2, and TUBB3 expressions in retinal-induced ASCs upon Notch signaling modulation treatments (, , and ).

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