Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro

Figure 3

Reactive oxygen and nitrogen species. Intracellular reactive oxygen species levels and nitric oxide levels were measured after 4 days incubation at 5% and 20% oxygen (a)–(c). No significant differences in ROS levels between 5% and 20% oxygen samples were observed (a). hAMSCs from placental amnion showed significantly lower levels of intracellular ROS (a). Calculation of correlation coefficient showed a strong negative association () between intracellular ROS levels and phosphorylation-related respiration (b). Increased nitric oxide release was detected in cell culture supernatants of samples incubated at 20% oxygen (c). (biological replicates), mean ± SD. Abbreviations: hAMSCs: human amniotic mesenchymal stromal cells; ROS: reactive oxygen species; RA: reflected amnion; P: placental amnion.

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