Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro

Figure 4

Release of immunoactive substances. Release of immunoactive substances in the cell culture supernatant of hAMSCs of reflected and placental amnion was measured after 4 days (5% and 20% oxygen) incubation (a)–(d). Higher oxygen tension (20%) lead to significantly higher secretion of IL-6 (a) and a trend to higher IL-10 release (b) in hAMSCs from placental amnion and had no effect on the release of hepatocyte growth factor (c) and IL1-beta release (d) compared to lower oxygen tension (5%). Cells from placental amnion released more IL-6 (a), IL-10 (b) and hepatocyte growth factor (c) when compared within cultures at same oxygen concentrations (5% and 20% oxygen). (biological replicates), mean ± SD. Abbreviations: hAMSCs: human amniotic mesenchymal stromal cells; IL: interleukin.

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