User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

Figure 3

The sequencing result of Eed knockout alleles. (a) Sequence of the LNL targeting locus in Eed after 4-OHT treatment. The LNL cassette was deleted by 4-OHT-inducible Cre-LoxP recombination, with one LoxP site being left. (b) Sequence of the FNFL targeting locus in Eed after Dox treatment. The FNFL cassette was deleted with one LoxP site and one FRT site being left. (c) Sequence of the Eed knockout allele generated by 4-OHT-inducible Cre-LoxP recombination. The LoxP site and FRT site are indicated by yellow and green boxes, respectively. The sequence alignment was analyzed by DNASTAR software. The additional mismatched nucleotides are the spacer between the LoxP/FRT site and the restriction endonuclease cut site used for homology arm construction as well as the spacer between LoxP and FRT sites in the FNFL cassette.

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