User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

Figure 6

Sequencing results of the conditional knockout allele of the 10 kb putative regulatory region. (a) Sequence of the FNFL targeting locus after Dox treatment. When FLP was induced by Dox, part of the FNFL cassette was deleted with one LoxP site and one FRT site left. The LoxP site and FRT site are indicated in yellow and green, respectively. (b) Sequence of the conditional knockout allele of the 10 kb regulatory region generated by 4-OHT treatment. The sequence alignment was analyzed by DNASTAR software.

(a)

(b)