User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

Table 1

Targeting efficiency of LNL and FNFL cassettes.

(a) Sequential targeting of LNL and FNFL cassettes

		Number of clones	Number of heteroinsertion	Number of homoinsertion	Efficiency of heteroinsertion	Efficiency of homoinsertion
Eed	LNL insertion	88	17	4	19.3%	4.5%
	FNFL insertion	190	33	5	17.4%	2.6%
SRCAP	LNL insertion	94	65	3	69.1%	3.2%
	FNFL insertion	96	70	2	72.9%	2.1%

(b) Simultaneous targeting of LNL and FNFL cassettes

		Clones with LNL heteroinsertion	Clones with LNL homoinsertion	Clones with FNFL heteroinsertion	Clones with FNFL homoinsertion	Clones with simultaneous LNL and FNFL homoinsertion
10 kb promoter	Number of clones; Total clone number :432	194	16	165	24	1
	Efficiency	44.9%	3.7%	38.2%	5.5%	0.2%