Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival

Figure 5

Expression of candidate stem cell and differentiation markers in association with age at diagnosis. (a) Illustration of the correlation analyses between mRNA expression of candidate stem cell marker (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) or potential differentiation marker (GFAP, Iba1, and Sparc) and patients’ age at diagnosis determined by Spearman’s nonparametric correlation in GBM samples. mRNA was analyzed by quantitative RT-PCR with normalization to 18S rRNA, . (b) Illustration of the correlation analyses between protein expression of candidate stem cell marker (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) or potential differentiation marker (GFAP, Iba1, and Sparc) and patients’ age at diagnosis determined by Spearman’s nonparametric correlation in GBM samples. Protein expression was determined by immunoblot analyses with normalization to GAPDH, no significant associations.

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