Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival

Figure 6

Association analysis of expression of candidate stem cell and differentiation markers with the gender of the GBM patients. (a) Illustration of the association analyses between mRNA expression of candidate stem cell marker (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) or potential differentiation marker (GFAP, Iba1, and Sparc) and the gender of GBM patients. Data are shown as box plots representing the median as horizontal bars as well as the 5th and 95th percentiles. mRNA level was analyzed by quantitative RT-PCR with normalization to 18S rRNA, Mann–Whitney U test, no significant differences. (b) Illustration of the association analyses between protein expression of candidate stem cell marker (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) or potential differentiation marker (GFAP, Iba1, and Sparc) and the gender of GBM patients. Data are shown as box plots representing the median as horizontal bars as well as the 5th and 95th percentiles. Protein expression was determined by immunoblot analyses with normalization to GAPDH, Mann–Whitney U test, no significant associations.

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