Research Article

Optimizing Osteogenic Differentiation of Ovine Adipose-Derived Stem Cells by Osteogenic Induction Medium and FGFb, BMP2, or NELL1 In Vitro

Figure 2

Immunophenotype of cells isolated from SVF conducted to be oADSCs. A gating of cells was based on FSC and SSC criteria, and the cells were subgated to only include single cells and only live cells were measured by fixable viability dye eFluor 506 staining and used subsequent analysis (data not shown). (a) CD45-negative cells were subgated to analyze the expression of (b) CD44 and CD29 and (c) CD31 and CD29. All quadrants were placed based on the isotype controls.
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