Research Article

Optimizing Osteogenic Differentiation of Ovine Adipose-Derived Stem Cells by Osteogenic Induction Medium and FGFb, BMP2, or NELL1 In Vitro

Figure 4

Quantification of the Alizarin red staining was made by hexadecylpyridinium chloride monohydrate (CC) after visualization. All wells were subtracted background from an average of 3 wells of 100 μl CC. Unpaired ANOVA was made on the mean values with SD of all wells and compared with Basic M. (basic medium) and OIM alone by Welch -test. indicates statistical significance () between basic medium and OIM group. indicates statistical significance () to OIM in a nonpaired -test between the two control groups.