The Anthrax Toxin Receptor 1 (ANTXR1) Is Enriched in Pancreatic Cancer Stem Cells Derived from Primary Tumor Cultures

Figure 2

ANTXR1/TEM8 is overexpressed in pancreatic CSCs. (a) Expression of TEM8 detected by Western blotting, in total lysates from L3.6pl or the indicated PDX-derived cell lines cultured in adherence (Adh) or as sphere (Sph). Tubulin was used as a loading control. Total HepG2 cell lysate was used a positive control (+ Ctl). (b) RTqPCR analysis of ANTXR1 relative mRNA expression levels in L3.6pl or the indicated PDX-derived cell lines cultured in adherence (Adh) or as sphere (Sph). mRNA expression levels for each target gene are normalized to β-actin levels ( samples per group). Fold changes were calculated compared to Adh (set at 1.0). (c) Representative flow cytometric analysis of TEM8 staining in Panc215, PancB023, or Panc354 cells cultured in adherence (Adh) or as sphere (Sph). Shown are the percent-positive cells present within the single-cell live debris-free population. (d) Histogram summarizing the percent of ANTXR1-postive cells present in L3.6pl or the indicated PDX-derived cell lines cultured in adherence (Adh) or as sphere (Sph). Fold changes were calculated compared to Adh (set at 1.0) (triplicate samples and experiments per cell line). , , and .

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