Research Article
Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues
Table 1
Specific equine primers used for the detection of endogenous and exogenous transcripts related to pluripotency and the reprogramming vector.
| Target | Sequence 5-3 | Fragment length (bp) | Reference |
| eOCT4 | F: TAGGGTTAGAGCTGCCCCCTC | 199 | XM_014734675.1 | R: GTTTGTGTTGTCCCTCCCCCA | eNANOG | F: ACTGCTCATTCAGGACAGCC | 200 | XM_014740545.1 | R: TCTGCTGGAGGCTGAGGTAT | eGAPDH | F: GTTTGTGATGGGCGTGAACC | 205 | NM_001163856.1 | R: ATCGCGCCACATCTTCCC | hSTEMCCA | F: AAGAGGACTTGTTGCGGAAA | 182 | Sommer et al., 2009 [23] | R: GGCATTAAAGCAGCGTATCC |
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