Stem Cells International / 2019 / Article / Fig 4

Research Article

Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities

Figure 4

Adipogenesis was revealed by Oil Red O staining for lipid droplet accumulation. (a) Representative images of PF ADSCs and BC ADSCs in control conditions and after adipogenic differentiation for 7 days (differentiated ADSCs). Scale bars: 200 μm. Data are representatives of five different isolates. (b) Magnifications of (a) images of PF ADSCs and BC ADSCs in control conditions and after adipogenic differentiation for 7 days (differentiated ADSCs). Accumulation of lipid droplets, stained with Oil Red O, is highlighted with red arrows. Scale bars: 100 μm. Data are representatives of five different isolates. (c) Percentage of cells positive to Oil Red O staining and quantification of Oil Red O extracted from lipid droplets measured at 520 nm normalized over nucleus counts. Data are reported as the of 5 different isolates. The statistical significance was determined by Student’s -test; and vs. UNDIFF PF ADSCs.
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