Bone Marrow-Derived CD44⁺ Cells Migrate to Tissue-Engineered Constructs via SDF-1/CXCR4-JNK Pathway and Aid Bone Repair

Table 3

Reagents used for in vitro experiments.

Reagent	Function	Application	Concentration	Duration	Source
CD44-PE Ab	Cell sorting	Flow cytometry	0.125 μg/test	30 min	eBioscience, USA
CD44 Ab	Cell tracing	Immunofluorescence	1 : 250	12 h	Abcam, Cambridge, UK
SDF-1	Proinflammatory cytokine	Transwell	100 ng/ml	24 h	PeproTech, USA
AMD3100	CXCR4 antagonist	Transwell	5 μg/ml	30 min	Sigma-Aldrich, USA
SP600125	JNK1/2/3 inhibitor	Transwell	10 μM	24 h	Sigma-Aldrich, USA
CXCR4 Ab	Protein analysis	Western blot	2 μg/ml	24 h	Abcam, Cambridge, UK
p-ERK Ab		Western blot	1 μg/ml	24 h	Abcam, Cambridge, UK
p-P38 Ab		Western blot	2 μg/ml	24 h	Abcam, Cambridge, UK
p-JNK Ab		Western blot	1.594 μg/ml	24 h	Abcam, Cambridge, UK
Arpin Ab		Western blot	1 μg/ml	24 h	Abcam, Cambridge, UK
Arp2/3 Ab		Western blot	0.1 μg/ml	24 h	CST, USA