Stem Cells International / 2019 / Article / Fig 1

Research Article

The Potential Role of Quorum Sensing in Clonal Growth and Subsequent Expansion of Bone Marrow Stromal Cell Strains in Culture

Figure 1

Growth phases from a BM CFU-F to a BM-MSC colony. (a) Developmental stages of primary CFU-F from human BM stromal DiI-labelled clonal cell to colony in culture (A–C) as observed by fluorescence microscopy (). (b) Development of an individual colony in human BM aspirate culture as observed by light microscopy (). Time lapse microscopy of isolated clonal cells (white arrow) from BM after explant: cell division and migration (days 1-7); define territory and size of the forming colony; and subsequent proliferation abilities within the colony [days 7-15; (A–J)]. Establishment of complete colonies as demonstrated by Giemsa staining (c, d). Results are derived from 5 independent experiments and from 5 different donors. DiI: 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate; CFU-F: Colony-Forming Unit Fibroblast; RBCs: Red Blood Cells. All data shown are representative results derived from one of at least three independent experiments. Scale bars represent 40 μm, 80 μm, 200 μm, and 400 μm and 1cm as indicated.

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