The Potential Role of Quorum Sensing in Clonal Growth and Subsequent Expansion of Bone Marrow Stromal Cell Strains in Culture
Clonal growth depends on serum. Samples of clonal nucleated cells from unsorted human BM were plated and cultured in 100 mm dishes ( cell/cm2, ). Photographs of culture dishes showing proliferation of primary BM CFU-F, cultured in standard CFU-F growth medium supplemented with 20% FBS (a) or cultured in SDM and supplemented with EGF/PDGF (10 ng/ml) (c). After 2 weeks of culture, well-developed colonies were observed in serum-supplemented cultures (a, b). In contrast, no colony developed in SDM; single cells remained attached with an intact nucleus and an intact cytoplasm (arrows), for at least 2 weeks, as demonstrated by Giemsa staining, but did not proliferate or generate any clone in serum-free medium (c, d). Results are derived from 5 independent experiments and from 5 different donors. FBS: Fetal Bovine Serum; EGF: Epidermal Growth Factor; PDGF-BB: Platelet-Derived Growth Factor. Scale bars represent 50 μm, 100 μm, and 200 μm.
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